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GATAD2A Antibody #19388

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  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 70, 73
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GATAD2A Antibody recognizes endogenous levels of total GATAD2A protein. This antibody does not cross-react with GATAD2B protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro56 of human GATAD2A protein. Antibodies are purified by peptide affinity chromatography.

    Background

    GATAD2A and GATAD2B, also known as p66 alpha and p66 beta, are related members of the nucleosome remodeling and deacetylase (NuRD) complex (1). GATAD2A interacts with MBD2 to recruit the deacetylase core of the NuRD complex to repress target genes, a process that is enhanced by the sumoylation of GATAD2A at Lys30 and Lys487 (2-5). In addition to being sumoylated, GATAD2A has also been shown to be phosphorylated by AMPK in the memory formation process (6). Depletion of GATAD2A in order to reduce NuRD-dependent repression of key pluripotency genes is being explored in the context of induced pluripotent stem cell (iPSC) generation (7,8).

    Alternate Names

    FLJ20085; FLJ21017; GATA zinc finger domain containing 2A; GATA zinc finger domain-containing protein 2A; GATAD2A; Hp66alpha; p66 alpha; P66A; p66alpha; Transcriptional repressor p66-alpha

    For Research Use Only. Not for Use in Diagnostic Procedures.
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