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Sall4 (6E3) Mouse Monoclonal Antibody #19477

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  • IHC

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1
    Application Key:
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Immunohistochemistry (Paraffin) 1:100 - 1:400

    Storage

    Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 12 months when stored at 4°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Sall4 (6E3) Mouse Monoclonal Antibody recognizes endogenous levels of Sall4 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a partial recombinant protein with GST tag corresponding to human Sall4 protein.

    Background

    Members of the SALL gene family encode putative zinc finger transcription factors highly expressed during development (1). Sall4 is expressed very early in development with other pluripotency regulators, such as Oct-4 and Nanog (2). Recent studies suggest Sall4 works as a master regulator that controls its own expression and the expression of Oct-4 in a transcriptional regulation feedback loop governing stem cell pluripotency and stem cell fate (2,3). Immunohistochemical studies indicate that Sall4 is a sensitive and specific diagnostic marker for primary germ cell tumors and yolk sac tumors (4,5). Research studies have shown that Sall4 is constitutively expressed in acute myeloid leukemia (AML) and is a probable effector of the Wnt/β-catenin signaling pathway in this disease (6). In addition, mutations in SALL4 have been implicated in human malformation syndromes, including Duane-radial ray syndrome (Okihiro syndrome) and Acro-renal-ocular syndrome (7).

    Alternate Names

    dJ1112F19.1; DRRS; HSAL4; MGC133050; sal-like 4 (Drosophila); Sal-like protein 4; SALL4; spalt like transcription factor 4; Zinc finger protein 797; Zinc finger protein SALL4; ZNF797

    For Research Use Only. Not for Use in Diagnostic Procedures.
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