Cell Signaling Technology Logo - Extra Large
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NBR1 (E6Q3F) Rabbit Monoclonal Antibody #20145

Filter:
  • WB
  • IP
  • IF

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 140
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200
    Immunofluorescence (Immunocytochemistry) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NBR1 (E6Q3F) Rabbit Monoclonal Antibody recognizes endogenous levels of total NBR1 protein. This antibody is suitable for detecting NBR1 by immunofluorescence when it's expressed at moderate-to-high levels.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro597 of human NBR1 protein.

    Background

    Next to BRCA1 gene 1 (NBR1) protein is known for its encoding gene proximity to the BRCA1 tumor suppressor gene (1,2). N-terminal Phox and Bem1p (PB1) domains of NBR1 mediate its interaction with muscle specific titin kinase (3,4) and scaffolding protein p62 (4). NBR1 plays a role in selective autophagy by facilitating the autophagosomal degradation of ubiquitinated proteins independently and also in concert with p62 (5-11).

    Alternate Names

    1A1-3B; 1A13B; B-box protein; Cell migration-inducing gene 19 protein; FLJ55359; FLJ98272; IAI3B; KIAA0049; M17S2; Membrane component chromosome 17 surface marker 2; membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigen CA125); MIG19; migration-inducing protein 19; NBR1; NBR1 autophagy cargo receptor; neighbor of BRCA1 gene 1; Neighbor of BRCA1 gene 1 protein; Next to BRCA1 gene 1 protein; Protein 1A1-3B

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.