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PU.1 (E8I8L) Mouse Monoclonal Antibody (PE Conjugate) #20251

Filter:
  • F

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1 kappa
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to phycoerythrin (PE) under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated PU.1 (E8I8L) Mouse mAb #89136.

    Fluorescent Properties

    • ← Excitation: 565 nm ← Emission: 575 nm

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    PU.1 (E8I8L) Mouse Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total PU.1 protein. This antibody conjugate does not cross-react with other Ets family members.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp26 of human PU.1 protein.

    Background

    PU.1 is a member of the Ets family of transcription factors and activates target genes through the purine-rich PU-box (1). PU.1 plays a pivotal role in the differentiation of myeloid cells and lymphocytes and is expressed in several hematopoietic cells, including B lymphocytes, macrophages, neutrophils, mast cells, early erythroid cells, and megakaryocytes (1,2). The concentration of PU.1 is critical for both the determination of hematopoietic cell lineage and the regulation of differentiation versus stem cell proliferation (3,4). In addition, PU.1 activity is influenced by phosphorylation and interactions with other hematopoietic transcription factors. Phosphorylation of PU.1 at Ser146 by CK2 promotes binding to IRF-4 and synergistic activation through the immunoglobulin κ 3' enhancer (5). Treatment of pro-B cells with IL-3 leads to phosphorylation of PU.1 at Ser140, resulting in increased PU.1 activity and activation of the anti-apoptotic gene MCL-1 (6). GATA1 binding blocks PU.1 activity during erythroid cell development (7). Overexpression of PU.1 resulting from proviral insertion during Friend virus infection can induce erythroleukemia, while reduced expression has been associated with acute myeloid leukemia (8).

    Alternate Names

    31 kDa transforming protein; 31 kDa-transforming protein; hematopoietic transcription factor PU.1; OF; PU.1; SFPI1; SPI-1; SPI-1 proto-oncogene; SPI-A; SPI1; spleen focus forming virus (SFFV) proviral integration oncogene spi1; Transcription factor PU.1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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