Product Pathways - Development
Axin2 (76G6) Rabbit mAb #2151
|2151S||100 µl (10 western blots)||---||In Stock||---|
|2151||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human||Endogenous||95, 98||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Axin2 (76G6) Rabbit mAb detects endogenous levels of total Axin2 protein. It does not cross-react with Axin1.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro566 of human Axin2.
Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 interacts with APC, GSK-3β, Dvl, and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice (6). Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).
- Luo, W. and Lin, S.C. Neurosignals 13, 99-113.
- Salahshor, S. and Woodgett, J.R. (2005) J. Clin. Pathol. 58, 225-236.
- Zeng, X. et al. (2005) Nature 438, 873-877.
- Davidson, G. et al. (2005) Nature 438, 867-872.
- Zhang, Y. et al. (1999) J. Biol. Chem. 274, 35247-35254.
- Chia, I.V. and Costantini, F. (2005) Mol. Cell Biol. 25, 4371-4376.
- Jho, E.H. et al. (2002) Mol. Cell Biol. 22, 1172-1183.
- Lustig, B. et al. (2002) Mol. Cell Biol. 22, 1184-1193.
- Leung, J.Y. et al. (2002) J. Biol. Chem. 277, 21657-21665.
- Callow, M.G. et al. (2011) PLoS One 6, e22595. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.