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Evi-1 Antibody #2265

Filter:
  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 105, 145, 200
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Evi-1 Antibody detects endogenous levels of total Evi-1 and MDS1/Evi proteins.

    Species Reactivity:

    Human

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Dog

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Leu921 of human Evi-1. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Evi-1 (Ecotropic virus integration site 1) was originally identified as a common site of viral integration in murine myeloid leukemia. It is involved in human myeloid disorders through chromosome translocation and inversion (1) and is also implicated in solid tumor formation (2). Evi-1 is a zinc finger transcription factor which also plays an important role in animal development (3). It has many isoforms due to alternative usage of 5'-ends (4), alternative splicing (5), and intergenic splicing which results in the formation of a fusion protein with MDS1 in normal tissues (6).

    Alternate Names

    AML1-EVI-1; AML1-EVI-1 fusion partner; AML1-EVI-1 fusion protein; ecotropic viral integration site 1; Ecotropic virus integration site 1 protein homolog; EVI-1; EVI1; Histone-lysine N-methyltransferase MECOM; KMT8E; MDS1; MDS1 and EVI1 complex locus; MDS1 and EVI1 complex locus protein; MDS1 and EVI1 complex locus protein EVI1; MDS1 and EVI1 complex locus protein MDS1; MDS1-EVI1; MECOM; MGC163392; MGC97004; myelodysplasia syndrome 1 protein; myelodysplasia syndrome-associated protein 1; oncogene EVI1; PR domain 3; PRDM3; RUSAT2; zinc finger protein Evi1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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