Cell Signaling Technology Logo - Extra Large

AP-2 gamma Antibody #2320

Filter:
  • WB
  • IF

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 49
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:400 - 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    AP-2 gamma Antibody detects endogenous levels of total AP-2γ protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human AP-2γ. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Activator protein 2γ (AP-2γ) is a member of the developmentally-regulated transcription factor activator protein 2 family (1). Overexpression of p53 in human breast cancer cells increases AP-2γ mRNA and protein (2). In addition, p53 binds to the AP-2γ promoter, suggesting that AP-2γ is a target of p53. AP-2γ therefore may contribute to p53-mediated growth inhibition (2). Studies also found that AP-2γ increases p21 mRNA and protein levels and induces cell-cycle arrest in G1 phase (3). Overexpression of AP-2γ inhibits the growth of human breast cancer cells (3). Taken together, these findings suggest that AP-2γ functions as a tumor suppressor (3).

    Alternate Names

    Activating enhancer-binding protein 2 gamma; AP-2 gamma; AP2-gamma; AP2C; ERF1; estrogen receptor factor 1; hAP-2g; TFAP2C; TFAP2G; Transcription factor AP-2 gamma; transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma); transcription factor AP-2 gamma (activating enhancer-binding protein 2 gamma); Transcription factor ERF-1

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.