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mu-Crystallin/CRYM (F9K9H) Rabbit Monoclonal Antibody #23208

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  • WB
  • IP

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 34
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    mu-Crystallin/CRYM (F9K9H) Rabbit Monoclonal Antibody recognizes endogenous levels of total mu-Crystallin/CRYM protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human mu-Crystallin/CRYM protein.

    Background

    Ketimine reductase μ-crystallin (CRYM) is a cytosolic multifunctional protein primarily expressed in the brain, muscle, prostate, and kidney. CRYM is an NADPH-regulated thyroid hormone-binding protein that binds triiodothyronine (T3) with high affinity, regulating its intracellular availability and action (1). Altered CRYM expression has been linked to thyroid hormone-dependent malignancies, such as prostate cancer, where CRYM levels correlate with more aggressive disease and poor prognosis (2). Mutations in the CRYM gene have been linked to autosomal dominant non-syndromic deafness, likely through disruption of its T3 binding function during critical cochlear structural development (3). When not bound to T3, CRYM functions as a ketimine reductase within lysine degradation pathways, including the pipecolate pathway, the primary route of lysine degradation in adult mammalian brain (4,5). Loss or reduction of CRYM expression in the brain is associated with neuronal vulnerability and cell death, and it has been implicated in several neurological and neurodegenerative diseases, including Huntington’s disease and Alzheimer’s disease (6,7).

    Alternate Names

    CRYM; crystallin mu; crystallin, mu; DFNA40; Ketimine reductase mu-crystallin; Mu-crystallin homolog; NADP-regulated thyroid-hormone binding protein; NADP-regulated thyroid-hormone-binding protein; THBP; thiomorpholine-carboxylate dehydrogenase

    For Research Use Only. Not for Use in Diagnostic Procedures.
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