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IDE (F4P1J) Rabbit Monoclonal Antibody #23827

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  • WB

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 118
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    IDE (F4P1J) Rabbit mAb recognizes endogenous levels of total IDE protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human IDE protein.

    Background

    Insulin-degrading enzyme (IDE) is a highly conserved zinc metalloprotease responsible for clearing short bioactive peptides, including insulin, glucagon, amylin, and β-amyloid (Aβ). IDE has the highest affinity for insulin and plays a critical role in regulating insulin levels across various tissues in the body (1). The enzyme contains two domains, IDE-N and IDE-C, which together form a dynamic catalytic chamber essential for substrate recognition, selectivity, and processing (2,3). IDE deficiency or dysfunction has been linked to type 2 diabetes mellitus and hyperinsulinemia due to impaired insulin clearance, making IDE a potential therapeutic target for diabetes (4). IDE has also been implicated in AD pathogenesis due to substrate competition between insulin and Aβ, and may provide insight into the connection between diabetes and Alzheimer’s disease (AD) (5).

    Alternate Names

    Abeta-degrading protease; FLJ35968; IDE; insulin degrading enzyme; Insulin protease; Insulin-degrading enzyme; Insulinase; Insulysin

    For Research Use Only. Not for Use in Diagnostic Procedures.
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