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  3. USB1 (F4R5D) Rabbit Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

USB1 (F4R5D) Rabbit Monoclonal Antibody #25000

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  • WB
  • IP

    Product Specifications

    REACTIVITY H R Mk
    SENSITIVITY Endogenous
    MW (kDa) 25, 28
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    USB1 (F4R5D) Rabbit Monoclonal Antibody recognizes endogenous levels of total USB1 protein.

    Species Reactivity:

    Human, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro46 of human USB1 protein.

    Background

    U6 biogenesis 1 (USB1), also known as MPN1, is a 3’-5’ RNA exonuclease that removes uridines and adenosines from the 3’ end of U6 snRNA, creating a 2’, 3’-cyclic phosphate formation, which protects U6 snRNA from degradation by the nuclear exosome (1-3). USB1 measures the U6 oligo(U) tail by reading the A102 nucleotide position and pauses five uridine residues downstream (3). Loss of USB1 results in a rare condition called poikiloderma with neutropenia, where U6 snRNA is unstable and contains aberrantly long and oligoadenylated 3’ ends, resulting in splicing dysfunction (1,4,5).

    Alternate Names

    C16orf57; chromosome 16 open reading frame 57; CP057; FLJ13154; hUsb1; HVSL motif containing 1; HVSL1; hypothetical protein LOC79650; Mpn1; mutated in poikiloderma with neutropenia protein 1; PN; putative U6 snRNA phosphodiesterase; U six biogenesis 1; U6 snRNA biogenesis 1; U6 snRNA biogenesis phosphodiesterase 1; U6 snRNA phosphodiesterase; U6 snRNA phosphodiesterase 1; UPF0406 protein C16orf57; USB1

    Database Links

    UniProt ID: Q9BQ65


    Entrez-Gene Id: 79650


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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