PTMScan® Formyl Lysine Motif (For-K) Kit #25217
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Background
Unlike other acyl-modifications on proteins, such as acetyl or succinyl lysine, formyl lysine is not primarily derived from an analogous coenzyme A metabolite. Instead, formyl lysine can be derived from diverse molecules, including peroxynitrite, 3'-formylphosphate, trichloroethylene oxide, and formaldehyde, and this PTM has been proposed as a biomarker for formaldehyde inhalation (3-7). Due to the various chemicals that can induce formylation, users should carefully consider their experimental workflows to avoid introducing spurious formyl lysines that can confound bona fide endogenous formyl lysine sites (8). Previous studies have identified formyl lysine sites on tau, histones, other non-histone chromatin factors, and milk proteins (3,9-11). In the context of tau, one proposed effect of this PTM is polymerization inhibition (3).
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- Vana, L. et al. (2011) Biochemistry 50, 1203-12.
- Jiang, T. et al. (2007) Proc Natl Acad Sci USA 104, 60-5.
- Edrissi, B. et al. (2013) PLoS Genet 9, e1003328.
- Cai, H. and Guengerich, F.P. (2000) Chem Res Toxicol 13, 327-35.
- Edrissi, B. et al. (2013) Chem Res Toxicol 26, 1421-3.
- Osés-Prieto, J.A. et al. (2007) Mol Cell Proteomics 6, 181-92.
- Wisniewski, J.R. et al. (2008) Nucleic Acids Res 36, 570-7.
- LeRoy, G. et al. (2009) Mol Cell Proteomics 8, 2432-42.
- Milkovska-Stamenova, S. and Hoffmann, R. (2019) Amino Acids 51, 891-901.
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