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Cleaved Caspase-6 (Asp162) (F6J4I) Rabbit Monoclonal Antibody #27643

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  • WB
  • IP

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 18
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cleaved Caspase-6 (Asp162) (F6J4I) Rabbit Monoclonal Antibody recognizes endogenous levels of caspase-6 protein only when cleaved at Asp162. This site refers to the cleavage site in rodent producing the p18 subunit. The homologous cleavage site in human is at Asp179. Weak reactivity may be observed for full-length caspase-6 in some cell lines.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp162 of mouse caspase-6 protein.

    Background

    Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes (1,2). Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated (3). The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to apoptosis (4,5). One of the major targets for caspase-6 is the membrane-associated protein lamin A (6). The cleavage of this protein causes cell membrane malfunction, membrane blebbing, and eventual cell death.

    Alternate Names

    Apoptotic protease Mch-2; CASP-6; Casp6; caspase 6; Caspase-6; Caspase-6 subunit p11; Caspase-6 subunit p18; mCAS; mCASP-6; Mch2

    For Research Use Only. Not for Use in Diagnostic Procedures.
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