Cell Signaling Technology Logo - Extra Large
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Recoverin (F2O5Z) Rabbit Monoclonal Antibody #27693

Filter:
  • WB
  • IF

    Product Specifications

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa) 23
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Frozen) 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Recoverin (F2O5Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total recoverin protein.

    Species Reactivity:

    Mouse, Rat

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly175 of mouse recoverin protein.

    Background

    Recoverin (Rcv), a small 23-kDa protein, is a member of the neuronal calcium sensor (NCS) family (1,2). It senses calcium levels in neurons and is primarily located in the outer segments of photoreceptors within the vertebrate retina, where it participates in light adaptation via calcium-dependent inhibition of the enzyme rhodopsin kinase (RK) (1). Rcv, like other family members, contains four EF-hand motifs and a myristoyl chain on the N-terminus. Only two of the EF-hands (EF2 and EF3) bind Ca2+ across species (1). In darkness, Ca2+-bound Rcv inhibits G protein-coupled receptors (GPCRs) and thereby prevents visual pigment phosphorylation. Under bright light conditions, Ca2+ dissociates from Rcv and the protein translocates to the inner segment. By this, Rcv prolongs phototransduction and enhances visual sensitivity (3). Few reports have described patients with anti-recoverin antibody-positive encephalitis (4).

    Alternate Names

    23 kDa photoreceptor cell-specific protein; cancer associated retinopathy protein; Cancer-associated retinopathy protein; CAR; guanylate cyclase activator; MGC129468; OTTMUSP00000006130; Protein CAR; Rcv1; Rcvrn; RECO; Recoverin; S-mo; S-modulin

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.