Cell Signaling Technology Logo - Extra Large
Trial Size Available Flag

CDK7 (MO1) Mouse Monoclonal Antibody #2916

Filter:
  • WB
  • IP
  • IHC

    Product Specifications

    REACTIVITY H R
    SENSITIVITY Endogenous
    MW (kDa) 40
    Source/Isotype Mouse IgG2b
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:2000
    Immunoprecipitation 1:200
    Immunohistochemistry (Paraffin) 1:500

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CDK7 (MO1) Mouse Monoclonal Antibody detects endogenous levels of total CDK7 protein. This antibody does not cross-react with other CDKs.

    Species Reactivity:

    Human, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant human CDK7. The epitope corresponds to a region surrounding Gln320 of human CDK7.

    Background

    CDK-activating kinase (CAK) is a complex of CDK7 and cyclin H. The complex is involved in cell cycle regulation by phosphorylating an activating residue in the T-loop domain of cdks (1). Regulation of CAK activity is mediated by T-loop phosphorylation and by association with MAT1, both of which enhance its kinase activity toward the CTD of RNA polymerase II (2,3) and other substrates such as p53 (4). CAK is an essential component of the transcription complex TFIIH and may interact directly with TFIIH helicases (5).

    Alternate Names

    39 kDa protein kinase; CAK; CAK1; CDK-activating kinase; CDK-activating kinase 1; CDK7; CDKN7; Cell division protein kinase 7; cyclin dependent kinase 7; Cyclin-dependent kinase 7; cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase); HCAK; homolog of Xenopus MO15 Cdk-activating kinase; kinase subunit of CAK; MO15; p39 Mo15; p39MO15; serine/threonine kinase stk1; serine/threonine protein kinase 1; serine/threonine protein kinase MO15; Serine/threonine-protein kinase 1; STK1; TFIIH basal transcription factor complex kinase subunit

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.