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Erlin-2 Antibody #2959

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  • WB
  • IP

    Product Specifications

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 43
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Erlin-2 Antibody detects endogenous levels of human erlin-2 protein.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln315 of mouse erlin-2. Antibodies are purified by peptide affinity chromatography.

    Background

    Erlin-1 and erlin-2 (SPFH1 and SPFH2) are SPFH domain-containing proteins that belong to the prohibitin family (1,2). The N-termal domain of erlin proteins contains ER-targeting sequences responsible for their translocation to the endoplasmic reticulum (ER) (3). In the ER, erlin-1 and erlin-2 specifically associate with the detergent resistant lipid raft microdomain of the membrane (3). Erlin-1 may be involved in dentritic cell activation (4) and erlin-2 has been shown to regulate the ER-associated degradation (ERAD) pathway by interacting with endogenous substrates and resulting in their polyubiquitination and degradation (5).

    Alternate Names

    C8orf2; Endoplasmic reticulum lipid raft-associated protein 2; epididymis secretory sperm binding protein; ER lipid raft associated 2; Erlin-2; ERLIN2; ERLN2; MGC87072; NET32; spastic paraplegia 18 (autosomal dominant); SPFH domain family, member 2; SPFH domain-containing protein 2; SPFH2; SPG18; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2

    For Research Use Only. Not for Use in Diagnostic Procedures.
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