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Ghrelin (D4K1W) Rabbit Monoclonal Antibody #31865

Filter:
  • IF

    Product Specifications

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Immunofluorescence (Frozen) 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #77865.

    Protocol

    Specificity / Sensitivity

    Ghrelin (D4K1W) Rabbit Monoclonal Antibody recognizes endogenous levels of total ghrelin protein. This antibody detects acylated (n-octanoyl) and non-acylated (desacyl) forms of ghrelin (confirmed by Western blot analysis of synthetic ghrelin protein).

    Species Reactivity:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse ghrelin protein.

    Background

    Ghrelin, also known as appetite-regulating hormone, is a neuropeptide hormone belonging to the motilin family. It is the ligand for the growth hormone secretagogue receptor type 1 (GHS-R), expressed by cells in the hypothalamic ventromedial nucleus and arcuate nucleus (1). Ghrelin is synthesized as a preprohormone by ghrelinergic cells in the gastrointestinal tract; proteolytic cleavage yields a 28-amino acid peptide hormone, which undergoes obligate n-octanoylation at serine 3 by the enzyme ghrelin O-acetyltransferase (GOAT) (2). Binding of n-octanoyl ghrelin to GHS-R stimulates growth hormone release, while simultaneously exerting multiple neuroendocrine affects that influence appetite, gastric motility and energy balance (3).

    Alternate Names

    2210006E23Rik; Appetite-regulating hormone; Ghr; Ghrelin; Ghrl; Growth hormone secretagogue; Growth hormone-releasing peptide; m46; Motilin-related peptide; Mtlrp; MTLRPAP; Obestatin; preproghrelin; proghrelin; Protein M46

    For Research Use Only. Not for Use in Diagnostic Procedures.
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