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VCAM-1 (D2T4N) Rabbit Monoclonal Antibody #32653

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  • WB
  • IHC

    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 85-110
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    VCAM-1 (D2T4N) Rabbit Monoclonal Antibody recognizes endogenous levels of total VCAM-1 protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant mouse VCAM-1 protein.

    Background

    VCAM-1 (vascular cell adhesion molecule-1) is a transmembrane glycoprotein containing multiple amino-terminal extracellular Ig-like domains, a transmembrane domain, and a short carboxy-terminal cytoplasmic domain (1). Alternative splicing generates two isoforms of VCAM-1 (2). The role of VCAM-1 during infection and inflammatory diseases is well characterized. Expression of VCAM-1 is induced in endothelial cells by inflammatory cytokines including TNF-α and IL-1β (1). VCAM-1 on endothelial cells interacts with the integrin VLA-4 (α4β1) on leukocytes to mediate migration of circulating leukocytes from the blood across the endothelium and into tissues (3).

    Alternate Names

    CD106; V-CAM 1; vascular cell adhesion molecule 1; Vascular cell adhesion protein 1; Vcam; VCAM-1; Vcam1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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