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GOT1 (E4A4O) Rabbit Monoclonal Antibody #34423

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  • WB

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 41
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GOT1 (E4A4O) Rabbit Monoclonal Antibody recognizes endogenous levels of total GOT1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala344 of human GOT1 protein.

    Background

    Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the interconversion of aspartate and oxaloacetate (1). The increased transamination primarily catalyzed by GOT1 leads to elevated levels of 2-hydroxyglutarate, which promotes methylation of the Foxp3 gene locus, inhibits Foxp3 expression and activates T helper 17 (TH17) cell differentiation (2). In addition, GOT1 is critical to the survival of cells with electron transport chain inhibition by generating aspartate, a metabolite determining the proliferation of these cells (3-4). Studies also show that GOT1 plays a key role in the noncanonical glutamine pathway that supports liver tumorigenesis (5).

    Alternate Names

    AATC; aspartate aminotransferase 1; Aspartate aminotransferase, cytoplasmic; aspartate transaminase 1; AST1; ASTQTL1; cAspAT; cCAT; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; GIG18; Glutamate oxaloacetate transaminase 1; glutamic-oxaloacetic transaminase 1; glutamic-oxaloacetic transaminase 1, soluble; glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1); GOT1; growth-inhibiting protein 18; testis secretory sperm-binding protein Li 196a; Transaminase A

    For Research Use Only. Not for Use in Diagnostic Procedures.
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