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Render Timestamp:
5/20/2026, 6:16:37 AM EDT
5/20/2026, 10:16:37 AM UTC
Commit: 7ed46ecc04b401f23a28df741b5078df405d23e4
XML generation date: 2026-05-13 07:53:46.168
Product last modified at: 2026-05-13T08:58:37.310Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

FoxA1/HNF3 alpha (E7E8W) Rabbit Monoclonal Antibody (Biotinylated) #35269

Filter:
  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 50
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is the biotinylated version of the unconjugated FoxA1/HNF3 alpha (E7E8W) Rabbit Monoclonal Antibody #53528 and is expected to exhibit the same species cross-reactivity. The concentration of the biotinylated antibody is 500 ug/ml. Peptide ELISA data were generated using the biotinylated antibody. Western blot data were generated using the unconjugated antibody.
    MW (kDa) 50

    Product Usage Information

    Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET.

    Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution.
    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    FoxA1/HNF3 alpha (E7E8W) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total FoxA1/HNF3α protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human FoxA1/HNF3α protein.

    Background

    Forkhead box protein A1 (FoxA1, HNF3α) is a transcription factor required for the development of endoderm-derived organs, such as liver, lung, and prostate (1). FoxA1 functions as a pioneer factor that is recruited primarily to the distant enhancers to change chromatin structure for transcription in a cell type-specific manner (2,3). The FoxA1 transcription factor is implicated in various diseases, playing a role in hormone-dependent disorders, such as breast and prostate cancers (4). The treatment of relapsing-remitting multiple sclerosis patients with IFN-β results in FoxA1-induced stimulation of a novel population of FoxA1(+) regulatory T cells, suggesting a possible immunosuppressive role for FoxA1 (5).

    Alternate Names

    forkhead box A1; Forkhead box protein A1; FOXA1; Hepatocyte nuclear factor 3-alpha; hepatocyte nuclear factor 3, alpha; HNF-3-alpha; HNF-3A; HNF3A; MGC33105; TCF-3A; TCF3A; Transcription factor 3A

    For Research Use Only. Not for Use in Diagnostic Procedures.
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