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AceCS2/ACSS1 (E6T5E) Rabbit Monoclonal Antibody #37041

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  • WB

    Product Specifications

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 75
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    AceCS2/ACSS1 (E6T5E) Rabbit Monoclonal Antibody recognizes endogenous levels of total AceCS2/ACSS1 protein. This antibody does not cross-react with AceCS1/ACSS2 protein.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly370 of human AceCS2/ACSS1 protein.

    Background

    Mitochondrial acetyl-CoA synthetase 2 (AceCS2), also known as acyl-CoA synthetase short-chain family member 1 (ACSS1), catalyzes acetyl-CoA biosynthesis from acetate and CoA. Studies show that acetate levels go up dramatically at sites of infection and inflammation, leading to the decrease of acetate assimilating enzymes AceCS2/ACSS1 and AceCS1/ACSS2 levels in memory CD8+ T cells. Acetate not metabolized by these enzymes instead stimulates glutaminase activity in memory CD8+ T cells, enhancing glutaminolysis, mitochondrial respiration, and cell survival (1). In addition, AceCS2/ACSS1 is highly expressed in one of the hepatocellular carcinoma (HCC) subtypes (2).

    Alternate Names

    ACAS2L; ACECS1; AceCS2; AceCS2L; Acetate--CoA ligase 2; Acetyl-CoA synthetase 2; acetyl-Coenzyme A synthetase 2 (AMP forming)-like; Acetyl-coenzyme A synthetase 2-like, mitochondrial; ACS2L; ACSS1; acyl-CoA synthetase short chain family member 1; Acyl-CoA synthetase short-chain family member 1; FLJ45659; KIAA1846; MGC33843; Propionate--CoA ligase

    For Research Use Only. Not for Use in Diagnostic Procedures.
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