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LAMP3/CD208 (E8T2T) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate) #37944

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  • IHC

    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated LAMP3/CD208 (E8T2T) Rabbit mAb #33401.

    Fluorescent Properties

    • ← Excitation: 495 nm ← Emission: 519 nm

    Product Usage Information

    Application Dilution
    Immunohistochemistry (Paraffin) 1:50 - 1:200

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    LAMP3/CD208 (E8T2T) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total LAMP3/CD208 protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn257 of mouse LAMP3/CD208 protein.

    Background

    Lysosome-associated membrane proteins (LAMPs) are a family of proteins present predominantly on the membrane of lysosomes. Five members have been identified so far (LAMP1, LAMP2, LAMP3, CD68/Macrosialin/LAMP4, and BAD-LAMP/LAMP5). LAMP proteins are involved in many different aspects of cell biology and can influence cellular processes, such as phagocytosis, autophagy, lipid transport, and aging. Their expression varies among different tissues (1). LAMP3, also known as CD-LAMP and CD208, is highly expressed only in certain cell types and differentiation stages. LAMP3 is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as a marker of human DC maturation, but is not expressed by DCs in mouse (2-4). Its expression is associated with poor prognosis of certain tumors, and the locus where it is encoded was identified as a risk factor for Parkinson's disease (PD) (5).

    Alternate Names

    1200002D17Rik; Cd208; DC LAMP; DC-L; DC-LAMP; DC-lysosome-associated membrane glycoprotein; DCLAMP; dendritic cells-lysosome associated membrane glycoprotein; LAMP; LAMP-3; Lamp3; Lysosomal-associated membrane protein 3; Lysosome-associated membrane glycoprotein 3; TSC40; TSC403

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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