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FANCM (E5Y9H) Rabbit Monoclonal Antibody #38199

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  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 230
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    MW (kDa) 230

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    FANCM (E5Y9H) Rabbit Monoclonal Antibody recognizes endogenous levels of total FANCM protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human FANCM protein.

    Background

    Fanconi anemia (FA) is an autosomal recessive genetic disorder resulting in symptoms that include chromosomal breakage, bone marrow failure, hypersensitivity to DNA cross-linking agents (such as mitomycin C), and a predisposition to cancer (1). FANCB is an X-linked member of the FA nuclear complex (FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCM). In response to DNA damage, the FA nuclear complex induces mono-ubiquitination of FANCD2 and FANCI (2). FANCJ/BRIP1, FANCD1/BRCA2, and FANCN/PALB2 are then recruited to sites of DNA damage along with other DNA repair proteins. FA signaling is important in maintenance of chromosome stability and control of mitosis (3).

    Studies of FANCB knockout embryonic stem cells suggest a role for FANCB in the formation of Rad51 and FANCD2 foci at chromosomal sites of DNA damage (4).

    Alternate Names

    ATP-dependent RNA helicase FANCM; FA complementation group M; FAAP250; FANCM; Fanconi anemia complementation group M; Fanconi anemia group M protein; Fanconi anemia-associated polypeptide of 250 kDa; Fanconi anemia, complementation group M; KIAA1596; MGC176453; POF15; Protein FACM; Protein Hef ortholog; SPGF28

    For Research Use Only. Not for Use in Diagnostic Procedures.
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