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IRGB10 (F8M4V) Rabbit Monoclonal Antibody #39002

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  • WB
  • IP

    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 47
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    IRGB10 (F8M4V) Rabbit mAb recognizes endogenous levels of total IRGB10 protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro116 of mouse IRGB10 protein.

    Background

    Interferon-gamma-inducible GTPase 10 (IRGB10) is a member of a family of interferon-inducible GTPases, also known as p47 GTPases (1). In mice, there are 23 genes present in the p47 GTPase family; only two are found in human, neither of which are orthologs of mouse IRGB10 (2,3). In macrophages, IRGB10 expression is upregulated by IRF1 in response to infection by pathogenic bacteria, such as E. coli and F. novicida (4). Along with guanylate binding proteins (GBPs), IRGB10 binds to and disrupts bacterial cell membranes, exposing bacterial ligands such as lipopolysaccharide (LPS) and bacterial DNA (4). These ligands may then be recognized by sensors such as AIM2 and caspase-11, leading to inflammasome formation (4). Similarly, IRGB10 targets the fungal cell wall of A. fumigatus during infection to combat the pathogen (5). Interestingly, unlike in the context of bacterial and fungal infection, in which IRGB10 contributes to host defense, in the context of influenza A infection, IRGB10 facilitates viral replication; IRGB10-/- mice were more resistant to influenza A infection than wild-type mice (6).

    Alternate Names

    Gm12250; Interferon-gamma-inducible GTPase 10; Interferon-gamma-inducible p47 GTPase; Irgb; Irgb10; OTTMUSG00000005723; predicted gene 12250

    For Research Use Only. Not for Use in Diagnostic Procedures.
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