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SF3A1 (F8X7A) Rabbit Monoclonal Antibody #41169

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  • WB
  • IP

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 95, 120
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SF3A1 (F8X7A) Rabbit Monoclonal Antibody recognizes endogenous levels of total SF3A1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro537 of human SF3A1 protein.

    Background

    The mammalian splicing factor SF3a contains three primary subunits: SF3A1 (SF3a120), SF3A2 (SF3a66), and SF3A3 (SF3a60), which, along with SF3b, activate the U2 snRNP to form the pre-mRNA splicing complex (1,2). SF3A2 and SF3A3 interact with SF3A1, but not with each other, and SF3A3 plays a major role in recruiting SF3A1 to the complex (3). Each protein in the heterotrimer has been shown to be essential for mRNA splicing and cell viability (4). Notably, SF3a plays a role in growth factor signaling as SF3A1 can bind to ERα when phosphorylated at Ser118 to affect downstream splicing (5). SF3a also plays a role in the innate immune response, promoting inflammation by splicing the TLR adaptor Myd88. Furthermore, SF3a regulates several genes involved in innate immune signaling pathways in macrophages versus other pathways (6,7).

    Alternate Names

    pre-mRNA processing 21; pre-mRNA splicing factor SF3a (120 kDa subunit); PRP21; PRPF21; SAP 114; SAP114; SF3A1; SF3a120; spliceosome associated protein 114; Spliceosome-associated protein 114; splicing factor 3 subunit 1; Splicing factor 3A subunit 1; splicing factor 3a, subunit 1, 120kD; splicing factor 3a, subunit 1, 120kDa

    For Research Use Only. Not for Use in Diagnostic Procedures.
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