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  3. FMC63 scFv (F2Q8G) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

FMC63 scFv (F2Q8G) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate) #41242

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  • F

    Product Specifications

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated FMC63 scFv (F2Q8G) Rabbit Monoclonal Antibody #16563.

    Fluorescent Properties

    • ← Excitation: 495 nm ← Emission: 519 nm

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:1600
    Flow Cytometry (Live) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    FMC63 scFv (F2Q8G) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate) recognizes exogenously expressed levels of CARs containing an FMC63-derived scFv.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with an scFv derived from a murine Anti-CD19 monoclonal antibody, FMC63.

    Background

    Chimeric antigen receptor (CAR) T cell therapy utilizes synthetic receptors to redirect T cells to bind and eliminate tumor cells, bypassing traditional T cell receptor (TCR) signaling (1). The extracellular domain of conventional CARs consists of single-chain variable fragments (scFvs), which recognize and bind target antigens (1). CARs engineered to express the scFv of a murine Anti-CD19 antibody, FMC63, have shown clinical success in targeting CD19-expressing malignant B cells for degradation. This clinical success has led to several FMC63-based CARs being approved by the U.S. Food and Drug Administration (FDA) for the treatment of B-cell leukemia and lymphoma (2,3).

    Alternate Names

    B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; B4; CD19; CD19 antigen; CD19 molecule; CVID3; Differentiation antigen CD19; MGC12802; T-cell surface antigen Leu-12

    Database Links

    UniProt ID: P15391


    Entrez-Gene Id: 930


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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