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THEM2 Antibody #42713

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  • WB
  • IP

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 15
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    THEM2 Antibody recognizes endogenous levels of total THEM2 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human THEM2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    THEM2 is a homotetrameric fatty acyl–CoA thioesterase (1). THEM2 and PC-TP (phosphatidylcholine transfer protein), both enriched in liver, interact to form a complex (1). Cell membrane-bound phosphatidylcholines bind to PC-TP in the complex (1). The complex in turn inhibits IRS-2 and mTORC1, which leads to the suppression of insulin signaling (1). THEM2 has also been shown to regulate adaptive thermogenesis in mice (2).

    Alternate Names

    ACO13; ACOT13; Acyl-CoA thioesterase 13; Acyl-coenzyme A thioesterase 13; Acyl-coenzyme A thioesterase 13, N-terminally processed; HT012; hypothalamus protein HT012; MGC4961; PNAS-27; THEM2; Thioesterase superfamily member 2

    For Research Use Only. Not for Use in Diagnostic Procedures.
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