Cell Signaling Technology Logo - Extra Large
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Layilin (E9U6M) Rabbit Monoclonal Antibody #42746

Filter:
  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 43, 55
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Layilin (E9U6M) Rabbit Monoclonal Antibody recognizes endogenous levels of total Layilin protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Layilin protein.

    Background

    Layilin (LAYN) is a single-pass type I integral membrane protein widely expressed by different cell types and tissues (1). LAYN is a hyaluronan receptor that directly interacts with several cytoskeletal-membrane linker molecules, including talin, merlin, and radixin (1-3). In both tumor and immune cell types, LAYN plays an important role in cell adhesion and motility, promoting cell spreading, migration, and infiltration (1,4-7). LAYN is involved in the tumor necrosis factor-α induced epithelial-mesenchymal transition of renal tubular epithelial cells (8), and also plays a critical role in HA35-induced intestinal epithelial tight junctions in inflammatory bowel disease (9). Lineage-specific deletion of LAYN on murine CD8+ T cells reduced their accumulation in tumors and increased tumor growth in vivo (10). Multiple isoforms have been identified.

    Alternate Names

    FLJ30977; FLJ31092; Layilin; LAYN

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.