Phospho-c-Myb (Ser11) (F6D5K) Rabbit Monoclonal Antibody #44427
- WB
- IP
- IHC
Product Specifications
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80-95 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
The c-Myb DNA-binding domain (DBD) can be phosphorylated at Ser11/Ser12 by casein kinase 2 (CK2), which similarly phosphorylates a-Myb and b-Myb, while it is uniquely phosphorylated by protein kinase A (PKA) (9,10). Ser11/Ser12 phosphorylation reduces c-Myb transactivation to target genes and alters its interaction with the cooperative transcription factor C/EBP beta (10). These inhibitory sites are frequently deleted in c-Myb-driven cancers (11), and c-Myb isoforms lacking the DBD are aberrantly expressed in adenoid cystic carcinomas through an alternative MYB promoter (12).
- Lin, H.H. et al. (1996) Curr Top Microbiol Immunol 211, 79-87.
- Mucenski, M.L. et al. (1991) Cell 65, 677-89.
- Badiani, P. et al. (1994) Genes Dev 8, 770-82.
- Cheasley, D. et al. (2011) Stem Cells 29, 2042-50.
- Thompson, M.A. et al. (1998) Cancer Res 58, 5168-75.
- Wilkins, H.R. et al. (2010) Tumour Biol 31, 16-22.
- Ramsay, R.G. and Gonda, T.J. (2008) Nat Rev Cancer 8, 523-34.
- Stenman, G. et al. (2010) Cell Cycle 9, 2986-95.
- Bergholtz S et al. (2001) Nucleic Acids Res 29, 3546-56.
- Oelgeschläger M et al. (1995) Mol Cell Biol 15, 5966-74.
- Lüscher B et al. (1990) Nature 344, 517-22.
- Frerich CA et al. (2019) Cancers (Basel) 12, 45. doi: 10.3390/cancers12010045.
Alternate Names
c-myb; c-myb protein (140 AA); c-myb_CDS; c-myb10A_CDS; c-myb13A_CDS; c-myb14A_CDS; c-myb8B_CDS; Cmyb; efg; MYB; MYB proto-oncogene, transcription factor; oncogene AMV; Proto-oncogene c-Myb; Transcriptional activator Myb; v-myb avian myeloblastosis viral oncogene homolog; v-myb myeloblastosis viral oncogene homolog (avian)
Limited Uses
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