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Chitotriosidase-1/CHIT1 (E4X4I) Rabbit Monoclonal Antibody #44627

Filter:
  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 50
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Chitotriosidase-1/CHIT1 (E4X4I) Rabbit Monoclonal Antibody recognizes endogenous levels of total Chitotriosidase-1/CHIT1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser406 of human Chitotriosidase-1/CHIT1 protein.

    Background

    Chitin is an essential biopolymer structural component of many organisms, including arthropods, fungi, and parasites. Chitinases are part of the 18-glycoside hydrolase (18-GH) family of proteins that act to bind and degrade chitin. In mammals, this function provides defense against chitin-containing pathogens. Chitotriosidase-1, also known as Chitinase-1 or CHIT1, is the most abundant of these enzymes. CHIT1 consists of an N-terminal catalytic domain, a C-terminal chitin-binding domain, and a short hinge region that connects the two. CHIT1 activity is implicated in several inflammatory signaling pathways and is considered a sensitive biomarker for activated immune cells. It is produced and secreted by macrophages, microglia, and neutrophils in normal and inflammatory conditions. Elevated levels of CHIT1 in serum is a suggested biomarker for Gaucher's disease, atherosclerosis, pulmonary fibrosis, and some cancers. Elevated CSF levels of CHIT1 is also a suggested biomarker for amyotrophic lateral sclerosis (ALS) and other neurodegenerative diseases (1-5).

    Alternate Names

    CHI3; CHIT; CHIT1; CHITD; chitinase 1; chitinase 1 (chitotriosidase); Chitinase-1; Chitotriosidase-1; FLJ00314; MGC125322; plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase

    For Research Use Only. Not for Use in Diagnostic Procedures.
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