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Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit Monoclonal Antibody #4514

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  • WB
  • F

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 44
    Source/Isotype Rabbit
    Application Key:
    • WB-Western Blotting 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Flow Cytometry (Fixed/Permeabilized) 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #75450.

    Protocol

    Specificity / Sensitivity

    Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit Monoclonal Antibody detects endogenous levels of SEK1/MKK4 phosphorylated at Ser257 .

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser257/Thr261 of human SEK1/MKK4.

    Background

    SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).

    Alternate Names

    c-Jun N-terminal kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 4; JNK activating kinase 1; JNK-activated kinase 1; JNK-activating kinase 1; JNKK; JNKK1; MAP kinase kinase 4; MAP2K4; MAPK/ERK kinase 4; MAPKK 4; MAPKK4; MEK 4; MEK4; mitogen-activated protein kinase kinase 4; MKK4; MP2K4; PRKMK4; SAPK kinase 1; SAPK/ERK kinase 1; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1; Stress-activated protein kinase kinase 1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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