MST4 (F5E7I) Rabbit Monoclonal Antibody #45277
- WB
- IP
- IF
- F
Product Specifications
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 52 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:800 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Mammalian sterile-20-like kinase 4 (MST4, STK26, MASK) is a Golgi-localized kinase that is cleaved by caspase-3 in vitro. While its potential role in apoptosis is unclear, research studies indicate that MST4 is involved in MAPK and EGF pathway signaling (5,6). MST4 and the serine/threonine kinase YSK1 (STK25) localize to the Golgi apparatus following association with the Golgi scaffold protein GM130. Binding to GM130 activates MST4 through autophosphorylation at Thr178 (7). MST4 contributes to liver homeostasis in several key pathways, including regulation of hepatocyte lipid droplet formation, ER stress, and oxidative stress (8,9). MST4, MST3, and YSK1 kinases have an overlapping set of liver-expressed substrates (ALOX5, GFAT1, MDM2, PKD, PRKFB3, Glut2, and NHE1) that may contribute to metabolic dysfunction–associated steatotic liver disease (MASLD) when these kinases are overexpressed (9). MST3/4 expression also correlates with the severity of hepatocellular carcinoma (HCC), and silencing either MST3 or MST4 can decrease HCC tumor growth and invasiveness (10).
- Dan, I. et al. (2001) Trends Cell Biol 11, 220-30.
- Creasy, C.L. et al. (1996) J Biol Chem 271, 21049-53.
- Lee, K.K. and Yonehara, S. (2002) J Biol Chem 277, 12351-8.
- Zhao, B. et al. (2011) Nat Cell Biol 13, 877-83.
- Dan, I. et al. (2002) J Biol Chem 277, 5929-39.
- Sung, V. et al. (2003) Cancer Res 63, 3356-63.
- Preisinger, C. et al. (2004) J Cell Biol 164, 1009-20.
- Caputo M et al. (2021) Hepatol Commun 5, 1183-1200.
- Cansby E et al. (2024) J Lipid Res 65, 100669.
- Caputo M et al. (2023) FASEB J 37, e23105.
Alternate Names
Mammalian STE20-like protein kinase 4; mammalian sterile 20-like 4; MASK; MST-4; Mst3 and SOK1-related kinase; MST4; serine/threonine kinase 26; serine/threonine protein kinase 26; serine/threonine protein kinase MASK; serine/threonine protein kinase MST4; Serine/threonine-protein kinase 26; Serine/threonine-protein kinase MASK; Serine/threonine-protein kinase MST4; STE20-like kinase 4; STE20-like kinase MST4; STK26
Limited Uses
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