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BRIP1/FANCJ Antibody #4578

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 145
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    BRIP1/FANCJ Antibody detects endogenous levels of human BRIP1/FANCJ protein.

    Species Reactivity:

    Human

    Source / Purification

    BRIP1/FANCJ Antibody is produced by immunizing rabbits with a synthetic peptide corresponding to amino acids near the carboxy terminus of human BRIP1/FANCJ. Antibodies are purified by Protein A and peptide affinity chromatography.

    Background

    BACH1, also known as BRIP1 and FANCJ, is a DNA helicase involved in repairing DNA cross-links and double-strand breaks (1-3). Interaction between phosphorylated BACH1 and BRCA1 is required for DNA damage-induced checkpoint signaling (3,4). Originally identified as a breast cancer susceptibility gene (1), the BACH1 gene is mutated in Fanconi anemia (5), a recessive disorder characterized by multiple congenital abnormalities, progressive bone marrow failure, and high cancer risk/predisposition. Research investigators have concluded that BACH1 interactions with BRCA1 and the presence of BACH1 mutations in patients with early-onset breast cancer indicate that BACH1 may act as a tumor suppressor (6).BACH1 phosphorylation at Thr1133 is thought to be involved in regulating the replication checkpoint and is required for BACH1 interaction with TopBP1 (7).

    Alternate Names

    ATP-dependent RNA helicase BRIP1; BACH1; BRCA1 interacting protein C-terminal helicase 1; BRCA1-associated C-terminal helicase 1; BRCA1-binding helicase-like protein BACH1; BRCA1-interacting protein 1; BRCA1-interacting protein C-terminal helicase 1; BRCA1/BRCA2-associated helicase 1; BRIP1; FANCJ; Fanconi anemia group J protein; FLJ90232; MGC126521; MGC126523; OF; Protein FACJ

    For Research Use Only. Not for Use in Diagnostic Procedures.
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