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  3. Phospho-Tau (Thr217) Quantitative Control

Phospho-Tau (Thr217) Quantitative Control #47640

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  • ELISA+

    Product Specifications

    MW (kDa) 70
    Application Key:
    • ELISA+-ELISA and/or ELISA-like Assays 

    Product Information

    Product Usage Information

    Prior to use, thaw the quantitative control and briefly spin down to collect all liquid at the bottom of the tube. Dilute according to the following instructions depending on the ELISA kit type. An example sensitivity curve is shown in Figure 2.

    PathScan® ELISA Kits:
    Dilute with ELISA Sample Diluent to achieve a 1X protein concentration.

    PathScan® RP ELISA Kits:
    Dilute with 1X Cell Lysis Buffer #9803 to a 2X protein concentration to achieve a final 1X protein concentration upon addition of the detection antibody.

    FastScan™ ELISA Kits:
    Dilute with 1X FastScan™ ELISA Cell Extraction Buffer to a 2X protein concentration to achieve a final 1X protein concentration upon addition of the antibody cocktail.

    Formulation

    Supplied in 1X PBS (8.1 mM Na2HPO4, 2.68 mM KCl, 1.675 mM KH2PO4, and 137 mM NaCl (pH 7.4)), 0.4% BSA, and 0.25% ProClin.

    Storage

    Store at -20°C.

    Product Description

    Phospho-Tau (Thr217) Quantitative Control is a peptide based standard specific for PathScan® RP Phospho-Tau (Thr217) Sandwich ELISA Kit #59672 and Phospho-Tau (Thr217) Matched Antibody Pair II #51525.
    MW (kDa) 70
    Purity Assessed to be > 90% by densitometry using SDS-PAGE analysis and Coomassie Blue detection.

    Background

    Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

    Database Links

    UniProt ID: P10636-8


    Entrez-Gene Id: 4137


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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