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Phospho-LYN (Tyr397)/LCK (Tyr394)/HCK (Tyr411)/BLK (Tyr389) (E5L3D) Rabbit Monoclonal Antibody (SignalFlex Alexa Fluor® 555 Conjugate) #47915

    Product Specifications

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and formulated at 200 µg/mL. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated #70926

    Fluorescent Properties

    • ← Excitation: 555 nm ← Emission: 565 nm

    Product Usage Information

    SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.

    Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Specificity / Sensitivity

    Phospho-LYN (Tyr397)/LCK (Tyr394)/HCK (Tyr411)/BLK (Tyr389) (E5L3D) Rabbit mAb (SignalFlex™ Alexa Fluor® 555 Conjugate) detects endogenous levels of LYN, LCK, HCK, and BLK only when phosphorylated at Tyr397, Tyr394, Tyr411, and Tyr389, respectively. It may cross react with overexpressed phosphorylated Src, YES, FYN, and FGR.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human LYN protein.

    Background

    The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr530 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

    Alternate Names

    ASV; c-SRC; c-syn protooncogene; c-yes; cellular yes-1 protein; FLJ26625; FYN; FYN oncogene related to SRC, FGR, YES; FYN proto-oncogene, Src family tyrosine kinase; HCK; HCK proto-oncogene, Src family tyrosine kinase; Hematopoietic cell kinase; Hemopoietic cell kinase; HsT441; IMD22; JTK8; JTK9; LCK; LCK proto-oncogene, Src family tyrosine kinase; Lck/Yes-related novel protein tyrosine kinase; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte-specific protein tyrosine kinase; LYN; LYN proto-oncogene, Src family tyrosine kinase; MGC45350; OKT3-induced calcium influx regulator; p53Lyn; p56-LCK; p56(LSTRA) protein-tyrosine kinase; p56lck; p56Lyn; p59-Fyn; p59-HCK/p60-HCK; p59Hck; p60-Src; p61-Yes; p61Hck; pp58lck; pp60c-src; Protein YT16; protein-tyrosine kinase fyn; Proto-oncogene c-Fyn; Proto-oncogene c-Src; Proto-oncogene c-Yes; Proto-oncogene Lck; Proto-oncogene Syn; proto-oncogene tyrosine-protein kinase fyn; proto-oncogene tyrosine-protein kinase LCK; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene tyrosine-protein kinase Yes; protooncogene SRC, Rous sarcoma; SLK; SRC; SRC proto-oncogene, non-receptor tyrosine kinase; src-like kinase; src/yes-related novel; SRC1; SYN; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; THC6; tyrosine kinase p59fyn(T); tyrosine kinase pp60c-src; Tyrosine-protein kinase Fyn; Tyrosine-protein kinase HCK; Tyrosine-protein kinase Lck; Tyrosine-protein kinase Lyn; tyrosine-protein kinase SRC-1; Tyrosine-protein kinase Yes; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian); v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1; v-yes-1 Yamaguchi sarcoma viral related oncogene homolog; viral oncogene yes-1 homolog 1; Yamaguchi sarcoma oncogene; Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog; YES; YES proto-oncogene 1, Src family tyrosine kinase; YES1; YES1 proto-oncogene, Src family tyrosine kinase; YT16

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    SignalFlex is a trademark of Cell Signaling Technology, Inc.
    This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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