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MLH1 (ES05) Mouse Monoclonal Antibody #47954

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Inquiry Info. # 47954

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    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1
    Application Key:
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    IHC Leica Bond 1:100 - 1:400
    Immunohistochemistry (Paraffin) 1:50 - 1:200
    Immunofluorescence (Immunocytochemistry) 1:400 - 1:800

    Storage

    Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 12 months when stored at 4°C. Do not aliquot the antibody.

    Note: This product is only available in the format listed above. We are unable to provide this in a carrier free, custom formulation.

    Protocol

    Specificity / Sensitivity

    MLH1 (ES05) Mouse Monoclonal Antibody recognizes endogenous levels of MLH1 protein. Non-specific cytoplasmic staining was observed in pancreas by immunohistochemistry.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a prokaryotic recombinant protein corresponding to 210 amino acids of human MLH1 protein.

    Background

    Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-γ), PMS2 (MutL-α), or MLH3 (MutL-γ). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases, and DNA ligases (reviewed in 1). Inactivation of the MLH1 gene causes genome instability and predisposition to cancer (2-5). The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) (6). MLH1 also plays a role in meiotic recombination (7).

    Alternate Names

    COCA2; DNA mismatch repair protein Mlh1; FCC2; hMLH1; HNPCC; HNPCC2; MGC5172; MLH1; mutL homolog 1; mutL homolog 1, colon cancer, nonpolyposis type 2; mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli); MutL protein homolog 1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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