IL-8 (E5F5Q) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 555 Conjugate) #50255
Product Specifications
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and formulated at 200 µg/mL. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated #94407
Fluorescent Properties
- ← Excitation: 555 nm ← Emission: 565 nm
Product Usage Information
SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.
Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.
Storage
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
IL-8 is produced by a number of cell types, including monocytes, T cells, neutrophils, fibroblasts, endothelial cells, and others (1). In addition to its effects on neutrophils, IL-8 promotes angiogenesis, inhibits endothelial cell apoptosis, and promotes the proliferation of melanoma cells in an autocrine fashion (1). As a result, IL-8 may be a contributing factor to the development of certain cancers (1,2). There are two distinct receptors for IL-8, CXCR1 and CXCR2; both are G protein-coupled receptors (1). Ligand binding induces Ca2+ mobilization and activates the PI3K, PKC, Rho, and Rac pathways (1,3).
Alternate Names
(Ala-IL-8)77; (Ser-IL-8)72; alveolar macrophage chemotactic factor I; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; C-X-C motif chemokine 8; C-X-C motif chemokine ligand 8; Chemokine (C-X-C motif) ligand 8; CXCL8; emoctakin; GCP-1; GCP/IL-8 protein I; GCP/IL-8 protein II; GCP/IL-8 protein III; GCP/IL-8 protein IV; GCP/IL-8 protein V; GCP/IL-8 protein VI; GCP1; granulocyte chemotactic protein 1; IL-8; IL-8(1-77); IL-8(5-77); IL-8(6-77); IL-8(7-77); IL-8(8-77); IL-8(9-77); IL8; IL8/NAP1 form I; IL8/NAP1 form II; IL8/NAP1 form III; IL8/NAP1 form IV; IL8/NAP1 form V; IL8/NAP1 form VI; interleukin 8; Interleukin-8; LECT; LUCT; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF; MDNCF-a; MDNCF-b; MDNCF-c; MONAP; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; NAF; NAP-1; NAP1; Neutrophil-activating factor; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Protein 3-10C; SCYB8; small inducible cytokine subfamily B, member 8; T cell chemotactic factor; T-cell chemotactic factor; tumor necrosis factor-induced gene 1
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