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ERAP2 (F3W9R) Rabbit Monoclonal Antibody #50824

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    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 120
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ERAP2 (F3W9R) Rabbit Monoclonal Antibody recognizes endogenous levels of total ERAP2 protein. This antibody does not cross-react with ERAP1 protein. Nonspecific staining was observed in normal human adrenal gland by immunohistochemistry.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu140 of human ERAP2 protein.

    Background

    Endoplasmic reticulum aminopeptidase 1 and 2 (ERAP1 and ERAP2) are IFN-γ inducible peptidases that are inserted into the endoplasmic reticulum (ER) membrane as type II transmembrane proteins (1). ERAPs trim antigenic peptide precursors to the optimal length required for presentation on MHC class I molecules (1). ERAP1 favors cleaving peptides between 9-16 amino acids in length, whereas ERAP2 optimally trims peptides shorter than 10 amino acids (2,3). Genetic mutations in the ERAP2 gene are associated with several autoimmune conditions, including Crohn’s disease and ankylosing spondylitis (4,5). Interestingly, these same mutations provide resistance to respiratory infections such as pneumonia and the bubonic plague, providing insight as to how these autoimmune-associated mutations could have been naturally selected for in certain populations (4,5). Due to the critical role of ERAP proteins in immune function, small-molecule ERAP1 and ERAP2 inhibitors are being investigated for the treatment of various cancers and autoimmune disorders (6).

    Alternate Names

    Endoplasmic reticulum aminopeptidase 2; ERAP2; FLJ23633; FLJ23701; FLJ23807; L-RAP; Leukocyte-derived arginine aminopeptidase; LRAP

    For Research Use Only. Not for Use in Diagnostic Procedures.
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