Reptin/RuvBL2 (D8N3J) Rabbit Monoclonal Antibody (BSA and Azide Free) #52152
- WB
Product Specifications
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 48 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
For standard formulation of this product see product #12668
Storage
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
Reptin plays a role in modulating cellular responses to hypoxia. Hypoxia induced methylation of Reptin by the methyltransferase G9a leads to its recruitment to hypoxia responsive promoters, where it negatively regulates transcription of these genes (8). In addition to transcriptional regulatory roles, Reptin also participates in the telomerase biogenesis processes as part of the telomerase complex. Reptin is involved in DNA damage response as part of the TIP60 acetyltransferase complex that stimulates ATM kinase activity necessary for phosphorylation of proteins involved in both checkpoint activation and DNA repair (9,10).
- Jha, S. and Dutta, A. (2009) Mol Cell 34, 521-33.
- Gallant, P. (2007) Trends Cell Biol 17, 187-92.
- Huber, O. et al. (2008) Cancer Res 68, 6873-6.
- Kim, J.H. et al. (2005) Nature 434, 921-6.
- Bauer, A. et al. (2000) EMBO J 19, 6121-30.
- Wood, M.A. et al. (2000) Mol Cell 5, 321-30.
- Dugan, K.A. et al. (2002) Oncogene 21, 5835-43.
- Lee, J.S. et al. (2010) Mol Cell 39, 71-85.
- Venteicher, A.S. et al. (2008) Cell 132, 945-57.
- Sun, Y. et al. (2010) Cell Cycle 9, 930-6.
Alternate Names
48 kDa TATA box-binding protein-interacting protein; 48 kDa TBP-interacting protein; 51 kDa erythrocyte cytosolic protein; CGI-46; ECP-51; ECP51; erythrocyte cytosolic protein, 51-KD; INO80 complex subunit J; INO80J; Repressing pontin 52; REPTIN; Reptin 52; Reptin52; reptin52 protein; RuvB (E coli homolog)-like 2; RuvB like AAA ATPase 2; RuvB-like 2; RuvB-like 2 (E. coli); RUVB2; RUVBL2; RVB2; TAP54-beta; TBP-interacting protein, 48-KD; TIH2; TIP48; TIP49B; TIP60-associated protein 54-beta
Limited Uses
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