Cat. # | Size | Qty. | Price |
---|---|---|---|
52366S | 100 µl |
|
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | |
Source/Isotype | IgG2a |
Product Information
Application | Dilution |
---|---|
Immunohistochemistry (Paraffin) | 1:500 - 1:2000 |
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
RECOMMENDED DETECTION REAGENTS |
SignalStain® Boost IHC Detection Reagent (HRP, Mouse) #8125 | SignalStain® Boost IHC Detection Reagent (AP, Mouse) #31926 |
---|---|---|
COMPATIBLE CHROMOGEN |
SignalStain® DAB Substrate Kit #8059 | SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713 |
SignalStain® Vivid Purple Peroxidase Substrate Kit #96632 | SignalStain® Ultra Blue Alkaline Phosphatase Substrate Kit #12824 | |
SignalStain® Deep Black Peroxidase Substrate Kit #72986 | ||
SignalStain® Radiant Yellow Peroxidase Substrate Kit #69644 |
NOTE: Use of detection reagents other than those specified in this protocol may require further optimization of the primary antibody to account for the different sensitivities of the detection reagents.
posted February 2010
revised June 2020
Protocol Id: 280
Human
Monoclonal antibody is produced by immunizing animals with full-length human recombinant SERPINB3 protein.
SERPINB3 (Serpin Family B Member 3) or SCCA1 (Squamous Cell Carcinoma Antigen 1) belongs to the serpin family and inhibits papain-like cysteine proteases (1). SERPINB3 is unique to the other serpins, as it lacks inhibitory activity against serine proteases but has inhibitory activity against papain-like cysteine proteases cathepsins K, L, and S (1,2). It may function as an inhibitor to modulate the host immune response against tumor cells and can act as a UV-induced apoptosis inhibitor by suppressing the activity of c-Jun NH2-terminal kinase 1 (JNK1) (1,3). SERPINB3 is expressed in normal squamous epithelium and in several organs, including epithelium of the tongue, esophagus, uterine cervix and vagina, Hassall’s corpuscles of the thymus, and some areas of the skin (4). SERPINB3 has been found to be overexpressed and implicated in several types of cancer, including hepatocellular carcinoma (HCC), lung, ovarian, and breast cancer (5-8). It is also overexpressed and implicated in hepatoblastoma, autoimmune disorders, lung fibrosis, cholesteatoma, and skin disease (9-13).
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