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  3. Phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) (D27F4) Rabbit Monoclonal Antibody (BSA and Azide Free)
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) (D27F4) Rabbit Monoclonal Antibody (BSA and Azide Free) #52554

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  • WB

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 52, 60
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #8828. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #8828

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    Phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) (D27F4) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of SMAD2 protein when phosphorylated at Ser465 and Ser467. This antibody also recognizes endogenous levels of SMAD3 protein when phosphorylated Ser422 only or at both Ser423 and Ser425.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser465/467 of human SMAD2 protein.

    Background

    Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, and 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7 (1-5). Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity (6-8).

    Alternate Names

    DKFZp586N0721; DKFZp686J10186; hMAD-2; hMAD-3; hSMAD2; hSMAD3; HSPC193; HsT17436; JV15-2; JV18; JV18-1; LDS1C; LDS3; MAD homolog 2; MAD homolog 3; mad homolog JV15-2; mad protein homolog; Mad protein homolog; Mad-related protein 2; MAD, mothers against decapentaplegic homolog 2; MAD, mothers against decapentaplegic homolog 3; Mad3; MADH2; MADH3; MADR2; MGC22139; MGC34440; MGC60396; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 2; Mothers against DPP homolog 3; Sma- and Mad-related protein 2; SMA- and MAD-related protein 3; SMAD 2; SMAD 3; SMAD family member 2; SMAD family member 3; SMAD, mothers against DPP homolog 2; SMAD, mothers against DPP homolog 3; SMAD2; SMAD3

    Database Links

    UniProt ID: P84022 , Q15796


    Entrez-Gene Id: 4088 , 4087


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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