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EGF Receptor (L858R Mutant Specific) (43B2) Rabbit Monoclonal Antibody (Biotinylated)

EGF Receptor (L858R Mutant Specific) (43B2) Rabbit Monoclonal Antibody (Biotinylated) #5354

Filter:

WB
ELISA+

Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	175
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
ELISA+-ELISA and/or ELISA-like Assays

Species Cross-Reactivity Key:

H-Human

Product Information

Product Description

This Cell Signaling Technology^® antibody is the biotinylated version of the unconjugated EGF Receptor (L858R Mutant Specific) (43B2) Rabbit Monoclonal Antibody #3197 and is expected to exhibit the same species cross-reactivity. The concentration of the biotinylated antibody is 500 ug/ml. Peptide ELISA data were generated using the biotinylated antibody. Western blot data were generated using the unconjugated antibody.

MW (kDa)

175

Product Usage Information

Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET.

Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution.

Storage

Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

EGF Receptor (L858R Mutant Specific) (43B2) Rabbit Monoclonal Antibody (Biotinylated) detects endogenous levels of EGFR mutant L858R protein. This antibody may cross-react with wildtype EGFR and other HER family members when highly over-expressed. Careful titration of this antibody may be required to obtain optimal specificity.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to L858R mutant sequence of human EGF receptor.

Background

The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Research studies have shown that somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarcinomas that respond to EGFR inhibitors, such as gefitinib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.

Alternate Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EGF receptor; EGFR; Epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); epidermal growth factor receptor tyrosine kinase domain; erb-b2 receptor tyrosine kinase 1; ERBB; ERBB1; HER1; mENA; NISBD2; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Database Links

UniProt ID: P00533

Entrez-Gene Id: 1956

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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