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CD27 (E5E7H) Rabbit Monoclonal Antibody (PE Conjugate) #53589

Filter:
  • F

    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to phycoerythrin (PE) under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated CD27 (E5E7H) Rabbit mAb #67996.

    Fluorescent Properties

    • ← Excitation: 565 nm ← Emission: 575 nm

    Product Usage Information

    Application Dilution
    Flow Cytometry (Live) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    CD27 (E5E7H) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total CD27 protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala127 of mouse CD27 protein.

    Background

    CD27 (TNFRSF7) is a transmembrane protein of the TNF receptor superfamily (TNFRSF). It is mainly expressed on lymphoid cells (also on early hematopoietic precursor cells in mice) (1,2). CD27 is considered a phenotypic marker for memory B cells and is also used to identify B regulatory (Breg) cells (3,4). It is constitutively expressed on naïve CD4 and CD8 T cells, and its expression is further upregulated upon T cell activation. CD27 is one of the two most important co-stimulatory receptors for T cell priming (the other one is CD28). While CD28 co-stimulatory signal mainly triggers cell proliferation, CD27 co-stimulatory signal primarily promotes cell survival and differentiation (5,6). Upon binding to its ligand CD70, CD27 activates the NF-κB and JNK signaling pathways through TNFR-associated factors (TRAFs), the adaptor molecules that are associated with CD27 cytoplasmic tail domain. Upon activation, CD27 is shed from the cell surface, and soluble CD27 is used as a marker of T cell activation (7,8).

    Alternate Names

    CD antigen 27; Cd27; CD27 antigen; CD27L receptor; OTTMUSP00000029268; OTTMUSP00000029269; S15; S152; T-cell activation antigen CD27; Tnfr; Tnfrsf7; Tp5; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

    For Research Use Only. Not for Use in Diagnostic Procedures.
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