Cell Signaling Technology Logo - Extra Large
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

C17orf59 (D2B5X) Rabbit Monoclonal Antibody #54037

Filter:
  • WB

    Product Specifications

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 42
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    C17orf59 (D2B5X) Rabbit Monoclonal Antibody recognizes endogenous levels of total C17orf59 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu270 of human C17orf59 protein.

    Background

    mTORC1 kinase complex is a critical component to regulate cell growth in response to energy levels, growth factors, and amino acids (1-4). The GTPases RagA, RagB, RagC and RagD have been shown to interact with raptor in mTORC1 (3,4). A Ragulator complex, consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3, LAMTOR3/MAPKSP1, LAMTOR4/C7orf59 and LAMTOR5/HBXIP, associates with the Rag GTPases and recruit them to the surface of lysosomes (5, 6). These interactions lead to mTORC1 activation in response to amino acid signals (3-6). In addition, C17orf59 was shown to interact with Ragulator and disrupt the interaction between Ragulator and Rag GTPases (7). This disruption prevents mTOR from localizing to the lysosomal membrane and therefore inhibits mTORC1 activation (7).

    Alternate Names

    BLOC-1 related complex subunit 6; BLOC-1-related complex subunit 6; BORC6; BORCS6; C17orf59; FLJ20014; LOC54785; Lysosome-dispersing protein; Lyspersin; PRO2472

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.