Product Pathways - Protein Folding
HOP (D10E2) XP® Rabbit mAb #5670
|5670S||100 µl (10 western blots)||---||In Stock||---|
|5670P||40 µl (4 western blots)||---||In Stock||---|
|5670||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey, Bovine||Endogenous||64||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
* Product-specific protocol.
Specificity / Sensitivity
HOP (D10E2) XP® Rabbit mAb recognizes endogenous levels of total HOP protein. This antibody is not known to cross-react with other heat shock proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro391 of human HOP protein.
Western blot analysis of whole cell extracts from various cell lines using HOP (D10E2) XP® Rabbit mAb.
Flow cytometric analysis of untreated HeLa cells using HOP (D10E2) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).
Confocal immunofluorescent analysis of HeLa cells using HOP (D10E2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
HOP, also known as stress-induced phospho protein 1 (STIP), is a co-chaperone to the major heat shock proteins, Hsp70 and Hsp90, and appears in early receptor complexes (1,2). Through mutual binding to both Hsp70 and Hsp90, Hop functions as an adaptor that can integrate Hsp70 and Hsp90 interactions (3,4). HOP is an abundant and highly conserved protein which is composed of three tetratricopeptide repeat (TPR) domains (TPR1, TPR2a and TPR2b) and two DP repeat domains (DP1 and DP2), whose function has not been fully resolved (5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.