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HIF-2 alpha (E2N9W) Rabbit Monoclonal Antibody

HIF-2 alpha (E2N9W) Rabbit Monoclonal Antibody #57921

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Product Specifications

REACTIVITY	M R
SENSITIVITY	Endogenous
MW (kDa)	120
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

HIF-2 alpha (E2N9W) Rabbit Monoclonal Antibody recognizes endogenous levels of total HIF-2α protein. This antibody does not cross-react with HIF-1α protein.

Species Reactivity:

Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse HIF-2α protein.

Background

Hypoxia-inducible factor (HIF) is essential for the cellular response to hypoxia (1,2). Under normoxia conditions, the α subunit of HIF is ubiquitinated by von Hippel-Lindau (VHL) protein and is degraded in the ubiquitin/proteasome pathway (1,2). Hypoxia inhibits degradation of the α subunit, which leads to its stabilization (1,2). HIF, in turn, regulates the transcription of a variety of genes that respond to hypoxia conditions (1,2). There are several isoforms of the HIF α subunit (2). Studies have found that HIF-1α and HIF-2α expression is increased in some human cancers (2). HIF-1α has both pro- and anti-proliferative activities, whereas HIF-2α does not possess anti-proliferative activity (2). Therefore, HIF-2α likely plays an important role in tumorigenesis (2,3).

Alternate Names

bHLHe7; bHLHe73; endothelial PAS domain protein 1; Endothelial PAS domain-containing protein 1; EPAS-1; Epas1; HIF; Hif like protein; HIF-1-alpha-like factor; HIF-2; HIF-2-alpha; HIF-2alpha; HIF-related factor; HIF1 alpha-like factor; HIF1alpha-like factor; HIF2-alpha; Hif2a; HLF; HLF (HIF1alpha-like factor); HRF; hypoxia inducible transcription factor 2alpha; Hypoxia-inducible factor 2-alpha; mHLF; MOP2

Database Links

UniProt ID: P97481

Entrez-Gene Id: 13819

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