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SH2D1B/EAT-2 (E2P5P) Rabbit Monoclonal Antibody #58173

Filter:
  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 15
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SH2D1B/EAT-2 (E2P5P) Rabbit Monoclonal Antibody recognizes endogenous levels of total SH2D1B/EAT-2 protein. This antibody does not cross-react with SH2D1A/SAP protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly92 of human SH2D1B/EAT-2 protein.

    Background

    SH2 domain-containing protein 1B/EWS/FLI1-activated transcript 2 (SH2D1B/EAT-2) belongs to the SAP family of cytoplasmic signaling adaptor proteins. Structurally, SH2D1B/EAT-2 is highlighted by a single SH2 domain and a short C-terminus containing multiple tyrosine residues whose phosphorylation regulate SH2D1B/EAT-2 function (1,2). Research studies have demonstrated that SH2D1B/EAT-2 is recruited to multiple SLAM family receptors in a phosphotyrosine-dependent manner and that phosphorylation of its C-terminal tyrosine residues is required for control of downstream signaling (3-5). SH2D1B/EAT-2 expression has been detected in multiple hematopoietic cell lineages, including NK cells and macrophages (4,6). Indeed, in murine NK cells, tyrosine phosphorylation of SH2D1B/EAT-2 mediates inhibitory signaling downstream of 2B4/SLAMF4 to suppress effector functions (4).

    Alternate Names

    EAT-2; EAT2; EWS/FLI1-activated transcript 2; SH2 domain containing 1B; SH2 domain-containing molecule EAT2; SH2 domain-containing protein 1B; SH21B; SH2D1B

    For Research Use Only. Not for Use in Diagnostic Procedures.
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