Cell Signaling Technology Logo - Extra Large
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DDX21 (E9O2Q) Rabbit Monoclonal Antibody #59278

Filter:
  • WB
  • IF

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 100
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DDX21 (E9O2Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total DDX21 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala780 of human DDX21 protein.

    Background

    DDX21, also known as RNA helicase II/Gu, is an RNA helicase that plays important roles in transcription and ribosomal RNA (rRNA) processing (1-3). DDX21 associates with many RNA Polymerase I and II genes, including the direct binding to the rDNA locus to promote ribosome biogenesis. The helicase activity of DDX21 contributes to transcription by helping release P-TEFb from the 7SK small nuclear ribonucleoprotein (snRNP) complex (3). Nucleolar localization of DDX21 has been shown to be regulated through phosphorylation by JNK and interaction with c-Jun. Depletion of either c-Jun or JNK has shown to impair rRNA processing (4,5). DDX21 can also become acetylated by CBP, which reduces its activity. Deacetylation by SIRT7 increases DDX21 helicase activity to prevent R-loop accumulation and protect genomic stability (6). DDX21 can also form a complex with DDX1, DHX36, and TRIF to sense dsRNA, which in turn activates interferon response in innate immunity (7,8).

    Alternate Names

    DDX21; DEAD (Asp-Glu-Ala-Asp) box helicase 21; DEAD (Asp-Glu-Ala-Asp) box polypeptide 21; DEAD box protein 21; DEAD-box helicase 21; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21; DExD-box helicase 21; DKFZp686F21172; Gu protein; Gu-alpha; GUA; GURDB; Nucleolar RNA helicase 2; Nucleolar RNA helicase Gu; Nucleolar RNA helicase II; RH II/Gu; RH-II/GU; RH-II/GuA; RNA helicase II/Gu alpha

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.