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DGK alpha (E7T8H) Rabbit Monoclonal Antibody #60796

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  • WB
  • IP
  • F

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Flow Cytometry (Fixed/Permeabilized) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DGK alpha (E7T8H) Rabbit Monoclonal Antibody recognizes endogenous levels of total DGKα ​​​​​​​ protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu734 of human DGKα protein.

    Background

    Diacylglycerol kinase alpha (DGKA) is a type 1 diacylglycerol kinase that competes with protein kinase C to convert messenger diacylglycerol (DAG) to phosphatidic acid (1). Upon activation of this intracellular lipid kinase, DGKA rapidly relocates from the cytosol to the plasma membrane where it is involved in multiple cellular processes, including regulating lipid and glucose metabolism levels, cell growth, and cell signaling (2,3). Expression of DGKA is high in T lymphocytes, and detectable in endothelial cells, epithelial cells, fibroblasts, and oligodendrocytes (4). DGKA negatively regulates T cell receptor-dependent responses through phosphorylation of phospholipase C-derived DAG (5-7). In contrast, DGKA-dependent phosphatidic acid generation by phosphorylation of a pre-existing DAG pool is necessary for IL-2-dependent T cell proliferation (8). DGKA is frequently overexpressed in cancer, promoting tumor cell survival, proliferation, invasion, and metastasis (9-12). Due to its role in immunity and cancer, targeting DGKA represents a unique opportunity for therapeutic intervention in cancer (13).

    Alternate Names

    80 kDa diacylglycerol kinase; DAG kinase alpha; DAGK; DAGK1; DGK-alpha; DGKA; Diacylglycerol kinase alpha; diacylglycerol kinase, alpha 80kDa; Diglyceride kinase alpha; MGC12821; MGC42356

    For Research Use Only. Not for Use in Diagnostic Procedures.
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