CSTF2 (F3J2F) Rabbit Monoclonal Antibody #60948
- WB
- IP
- IF
Product Specifications
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
CSTF2, through its ability to directly bind RNA, has been implicated in many cellular functions and diseases. In embryonic stem cells, CSTF2 controls pluripotency and cell cycle through 3’-end processing of histone mRNAs (6). Widespread shortening of 3’-UTRs has been a phenomenon described in many cancer types. Overexpression of CSTF2 shortens the poly(A) tails of oncogenes, resulting in evasion of miRNA repression and transformation (7).
- MacDonald, C.C. et al. (1994) Mol Cell Biol 14, 6647-54.
- Rüegsegger, U. et al. (1996) J Biol Chem 271, 6107-13.
- McCracken, S. et al. (1997) Nature 385, 357-61.
- Kleiman, F.E. and Manley, J.L. (1999) Science 285, 1576-9.
- Kleiman, F.E. and Manley, J.L. (2001) Cell 104, 743-53.
- Youngblood, B.A. et al. (2014) Nucleic Acids Res 42, 8330-42.
- Mayr, C. and Bartel, D.P. (2009) Cell 138, 673-84.
Alternate Names
betaCstF-64 variant 2; CF-1 64 kDa subunit; Cleavage stimulation factor 64 kDa subunit; Cleavage stimulation factor subunit 2; cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kD; cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa; CSTF 64 kDa subunit; CstF-64; CSTF2
Limited Uses
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