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RUNX2 (D1L7F) Rabbit Monoclonal Antibody (SignalFlex mFluor UV375 Conjugate) #61925

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to mFluor™ UV375 fluorescent dye under optimal conditions and formulated at 200 µg/mL. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated #12556

    Fluorescent Properties

    • ← Excitation: 351 nm ← Emission: 387 nm

    Product Usage Information

    SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.

    Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Specificity / Sensitivity

    RUNX2 (D1L7F) Rabbit Monoclonal Antibody (SignalFlex™ mFluor™ UV375 Conjugate) recognizes endogenous levels of total RUNX2 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala273 of human RUNX2 protein.

    Background

    Runt-related transcription factor 2 (RUNX2) is a member of the RUNX family of transcription factors. It is involved in osteoblast differentiation and skeletal morphogenesis. RUNX2 regulates the transcription of various genes, including osteopontin, bone sialoprotein, and osteocalcin, via binding to the core site of the enhancers or promoters (1-3). RUNX2 is crucial for the maturation of osteoblasts and both intramembranous and endochondral ossification. Mutations in the corresponding RUNX2 gene have been associated with the bone development disorder cleidocranial dysplasia (CCD) (4-6). RUNX2 is also abnormally expressed in various human cancers, including prostate and breast cancer. It plays an important role in migration, invasion, and bone metastasis of prostate and breast cancer cells (7-10).

    Alternate Names

    Acute myeloid leukemia 3 protein; AML3; CBF-alpha 1; CBF-alpha-1; CBFA1; CCD; CCD1; CLCD; Core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; MGC120022; MGC120023; Oncogene AML-3; OSF-2; OSF2; Osteoblast-specific transcription factor 2; PEA2-alpha A; PEA2aA; PEBP2-alpha A; PEBP2A; PEBP2A1; PEBP2A2; PEBP2aA; PEBP2aA1; polyomavirus enhancer binding protein 2 alpha A subunit; Polyomavirus enhancer-binding protein 2 alpha A subunit; runt related transcription factor 2; Runt-related transcription factor 2; RUNX family transcription factor 2; RUNX2; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    mFluor is a trademark of AAT Bioquest, Inc.
    SignalFlex is a trademark of Cell Signaling Technology, Inc.
    mFluor is manufactured by AAT Bioquest, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.